Journal: Reproduction & Fertility

Article Title: Aspirin enhances endometrial decidualization markers in vitro among women with and without endometriosis

doi: 10.1530/RAF-25-0034

Figure Lengend Snippet: (A, B, C, D) Aspirin (ASA) enhances decidualization markers of endometrial stromal cells (ESCs). Treatment of ESCs with ASA (1–2.5 mM) prior to stimulation with either (A) cAMP alone or (B) cAMP + MPA enhances decidualization markers, as determined by IGFBP1 protein levels by ELISA when compared to vehicle (Veh)-treated ESCs. Treatment of ESCs with ASA (1–2.5 mM) prior to stimulation with either (C) cAMP alone or (D) cAMP + MPA enhances decidualization markers, as determined by PRL protein levels by ELISA compared to vehicle (Veh)-treated ESCs. Each dot represents data from ESCs isolated from one participant. Significance was determined by the Kruskal–Wallis test with post hoc Dunn’s multiple comparison test; P -values are shown. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: After 48 h, culture supernatants were collected after brief centrifugation and cell-free supernatants were analyzed for decidualization markers, IGFBP1 or PRL, using Human IGFBP1 and PRL DuoSet ELISA Kits (R&D Systems®, USA), respectively, according to the manufacturer’s directions and as previously reported ( Warren et al. 2018 , Nayyar et al. 2020 , Delenko et al. 2024 ).

Techniques: Enzyme-linked Immunosorbent Assay, Isolation, Comparison

Journal: Journal of Molecular Medicine (Berlin, Germany)

Article Title: Glis3 as a critical regulator of Pit1-lineages and renal functions

doi: 10.1007/s00109-025-02611-3

Figure Lengend Snippet: Hyperprolactinemia of glis3KD larvae. a Relative mRNA expression of prl CTRL, glis3KD larvae at 120 hpf. qRT-PCR data are normalized to eef1α , and the results are shown as mean ± SD of n = 3 independent replicates. Each data point represents a pool of 20 larvae. Student- t -test was used for statistical analysis between groups: *** P < 0.001. b ELISA of prolactin (ng/ml) in control and glis3KD larvae at 120 hpf. Results (mean ± SD of 3 pools, 50 larvae/pool) were analyzed by Student’s t-test: *** P < 0.001. c , c’ Representative FISH images of prl in CT and KD larvae at 120 hpf. All larvae were acquired by confocal microscopy in ventral view, head to the top. Scale bars in c = 100 µm. d Quantification of total cell volume (µm 3 ) of prl using confocal analysis on the same area (ROI) and number of sections for each embryo. Data are displayed as dot plots with mean ± SD of n = 15 embryos each. Mann–Whitney test was used for statistical analysis, *** P < 0.001. e – f’ IF of prolactin in control and glis3KD larvae in lateral ( e , e’ ) or ventral ( f , f’ ) view. Prl localized in gills (g, asterisks), pectoral fins (pf, arrow), pronephric ducts (pd, arrowheads), forebrain (fb, arrow), and adenohypophysis (ah, arrowhead). Scale bars: e = 300 µm; f = 200 µm. g Mean fluorescence intensity (MFI) of prolactin-positive tissues ( n = 15) using Fiji Software. Mann–Whitney test: ns, not significant; *** P < 0.001

Article Snippet: Prolactin levels (ng/ml) were measured using a Fish PRL ELISA Kit (CusaBio, CSB-E12695Fh).

Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Control, Confocal Microscopy, MANN-WHITNEY, Fluorescence, Software